Immunological Sciences

Laminin β-2 Rabbit Polyclonal Antibody

Western blot analysis of lysates from RAW264.7 cells, using LAMB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from RAW264.7 cells, using LAMB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-E9720
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human LAMB2. AA range:61-110
Reactivity:Human;Mouse;Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300
Molecular Weight:210kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:LAMB2; LAMS; Laminin subunit beta-2; Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin sub

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human LAMB2. AA range:61-110

Target Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1.

Synonyms:LAMB2; LAMS; Laminin subunit beta-2; Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin sub

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from RAW264.7 cells, using LAMB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbeD.

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