LAMP1 / CD107a Mouse Monoclonal Antibody

Immunohistochemistry staining of CD107a (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody H4A3 (anti-CD107a) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.

<b>Anti-Hu CD107a Purified (clone H4A3) works in WB application under reducing and non-reducing conditions.</b> Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of A431, THP-1, U87MG, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel. Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4A3 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed. CD107a molecules were detected at 120 kDa in all tested cell lines under both reducing and non-reducing conditions.

Flow cytometry analysis (intracellular staining) of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.

Western blot analysis of different cell lysates using mouse mAb to LAMP1, dilution 1:10,000 in green. Cells were maintained under normal conditions (Ct), or treated with 50

Immunofluorescent analysis of HeLa cells stained with mouse mAb to LAMP1, dilution 1:500 in red, and co-stained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The cells were treated with 50