Immunological Sciences

LAMP3 /CD63 Mouse Monoclonal Antibody

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - 547  cell nuclei stained with DAPI (blue)

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - 547  cell nuclei stained with DAPI (blue)

Catalog Number:MAB-10067
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:MEM-259
Immunogen:HPB-ALL T cell line
Reactivity:Human
Applications:Flow cytometry: Recommended dilution: 2 μg/ml; positive material: activated platelets, neutrophils and basophils. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
Molecular Weight:40-60 kDa
Purification:Purified by protein-A affinity chromatography.
Form:Liquid
Synonyms:OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

Applications

Flow cytometry: Recommended dilution: 2 μg/ml; positive material: activated platelets, neutrophils and basophils. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.

Immunogen

HPB-ALL T cell line

Target Background

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas

Synonyms:OMA81H, Granulophysin, Tetraspanin-30, Tspan-30, MLA1, ME491, LAMP-3, OMA81H, TSPAN30

Storage

Store at 2-8°C. Do not freeze.

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259; green) after co-incubation of living cells with human Transferrin - 547  cell nuclei stained with DAPI (blue)

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 .

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

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