LC3A/LC3B Rabbit Monoclonal Antibody
Western blot analysis of various lysates using LC3A/LC3B Rabbit Monoclonal Antibody at 1:10000 dilution incubated at room temperature for 1.5 hours. Hela and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours.
| Catalog Number: | AB27200 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:10000 - 1:20000; IHC-P 1:2000 - 1:8000; IF/ICC 1:200 - 1:800; ELISA Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 14kDa/16kDa |
| Purification: | Affinity purification |
| Synonyms: | LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; LC3A/B; LC3B |
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Applications
WB 1:10000 - 1:20000; IHC-P 1:2000 - 1:8000; IF/ICC 1:200 - 1:800; ELISA Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
