Immunological Sciences

LC3B Polyclonal Antibody

Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody at 1:1000 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody at 1:1000 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84447
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunohsitochemistry: 1:50 - 1:200 Immunofluorescence / Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Molecular Weight:14KDa/16KDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B

Applications

Western Blot: 1:500 - 1:2000 Immunohsitochemistry: 1:50 - 1:200 Immunofluorescence / Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B.

Target Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A andMAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Synonyms:LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of NIH/3T3 cells, using LC3B antibody at 1:1000 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts from wild type (WT) and [KO Validated] LC3B Rabbit mAb knockout (KO) 293T cells, using [KO Validated] LC3B Rabbit mAb at 1:1000 dilution.293T and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Immunohistochemistry of paraffinembedded human brain using [KO Validated] LC3B Rabbit mAb at dilution of 1:100(40x ens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of 293T Treated with Chloroquine and 293T cells using [KO Validated] LC3B Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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