Immunological Sciences

LexA DNA Binding Region Rabbit Polyclonal Antibody

No images available

Catalog Number:ABN00454
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Reactivity:Cyanobacteria
Applications:WB 1:500-1:1000
Molecular Weight:Refer to figures
Purification:Affinity Purification
Form:Liquid
Synonyms:lexa dna binding region

Applications

WB 1:500-1:1000

Target Background

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.

Synonyms:lexa dna binding region

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

No documents available.