LIM Kinase 2 Rabbit Polyclonal Antibody

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Catalog Number:ABN00460
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:A synthetic peptide of human LIM kinase 2
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:100-1:200
Molecular Weight:Calculated MW: 72 kDa; Observed MW: 72 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:LIM domain kinase 2

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:100-1:200

Immunogen

A synthetic peptide of human LIM kinase 2

Target Background

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics (PubMed:10436159, PubMed:11018042). Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, PubMed:11018042). Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP (PubMed:22328514). Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro (PubMed:8537403). Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).

Synonyms:LIM domain kinase 2

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

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