Immunological Sciences

LIN28B Mouse Monoclonal Antibody

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Catalog Number:MABN86094
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.
Reactivity:Human, Mouse
Applications:WB 1:1000-1:2000
Molecular Weight:27.1kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2

Applications

WB 1:1000-1:2000

Immunogen

This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.

Target Background

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Synonyms:Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide.

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