Immunological Sciences

LRP6

Western blot analysis of extracts of mouse liver, using LRP6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.

Western blot analysis of extracts of mouse liver, using LRP6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.

Catalog Number:AB-84243
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human LRP6
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:170kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:LRP6;ADCAD2;STHAG7

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human LRP6

Target Background

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptormediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.

Synonyms:LRP6;ADCAD2;STHAG7

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of mouse liver, using LRP6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 60s.

Immunohistochemistry of paraffin embedded rat heart using LRP6 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded human gastric cancer using LRP6 antibody at dilution of 1:100 (40x lens).

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