Immunological Sciences

MBD1 (3J6) Rabbit Monoclonal Antibody

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Catalog Number:MABN13680
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human MBD1
Reactivity:Human
Applications:WB 1:500-1:2000,IHC 1:100-1:200,ICC/IF 1:100-1:200
Molecular Weight:67kDa
Purification:Affinity purification
Form:Liquid
Synonyms:CXXC 3; Mbd1; MECP1 COMPLEX; PCM1; RFT;

Applications

WB 1:500-1:2000,IHC 1:100-1:200,ICC/IF 1:100-1:200

Immunogen

A synthetic peptide of human MBD1

Target Background

Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

Synonyms:CXXC 3; Mbd1; MECP1 COMPLEX; PCM1; RFT;

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

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