MDM2 Polyclonal Antibody
Western Blot analysis of Hela cells using MDM2 Polyclonal Antibody at dilution of 1:2000.
| Catalog Number: | AB31995 |
| Conjugate: | Unconjugated |
| Size: | 100μL |
| Concentration: | 1 mg/mL |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Synthesized peptide derived from human MDM2 around the non-phosphorylation site of Ser166. |
| Reactivity: | Human;Mouse;Monkey |
| Applications: | Western Blot: 1:500-1:2000, Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 |
| Molecular Weight: | 90 kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
Applications
Western Blot: 1:500-1:2000, Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000
Immunogen
Synthesized peptide derived from human MDM2 around the non-phosphorylation site of Ser166.