Immunological Sciences

Mesothelin Rabbit Polyclonal Antibody

Western blot analysis of lysates from OVCAR3 cells, using Mesothelin Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Western blot analysis of lysates from OVCAR3 cells, using Mesothelin Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-82814
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:1000 - 1:5000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200
Molecular Weight:46-48kDa/70kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MPF; SMRP; Mesothelin

Applications

Western Blot: 1:1000 - 1:5000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 296-606 of human Mesothelin

Target Background

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cellsurface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Synonyms:MPF; SMRP; Mesothelin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of lysates from OVCAR3 cells, using Mesothelin Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Immunohistochemistry analysis of Mesothelin in paraffin-embedded human lung cancer（200X）using Mesothelin Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Mesothelin in paraffin-embedded human lung cancer（400X） using Mesothelin Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Mesothelin in paraffin-embedded Human tonsil（200X）using Mesothelin Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Mesothelin in paraffin-embedded Human tonsil（400X）using Mesothelin Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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