Immunological Sciences

MET (c-Met)

Western blot analysis of extracts of various cell lines, using MET antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Western blot analysis of extracts of various cell lines, using MET antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:AB-83944
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human MET.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 -1:2000
Molecular Weight:155-180kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MET; AUTS9; DFNB97; HGFR; RCCP2; c-Met; hepato cyte growth factor receptor

Applications

Western Blot: 1:500 -1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human MET.

Target Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Synonyms:MET; AUTS9; DFNB97; HGFR; RCCP2; c-Met; hepato cyte growth factor receptor

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using MET antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

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