Immunological Sciences

MITF

Western blot analysis of extracts of various cell lines, using MITF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H +L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using MITF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H +L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:MAB-94447
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:DG7V5
Reactivity:Hu, Ms, Rt
Applications:Western blotting 1:500-1:1000
Molecular Weight:65 kDa
Purification:Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly335 of human MITF protein.
Form:Liquid

Applications

Western blotting 1:500-1:1000

Target Background

Microphthalmia-associated transcription factor (MITF) is a basic helix-loop-helix leucine zipper transcription factor that is most widely known for its roles in melanocyte, ophthalmic, and osteoclast development (1-3). In humans, MITF can function as a melanoma oncogene (4) and mutations in the corresponding MITF gene are associ-ated with Waardenburg syndrome type 2, an auditory-pig-mentary syndrome characterized by developmental defects in cells derived from neural crest (5). At least 12 isoforms of MITF have been identified, which exhibit differential patterns of expression of cell and tissue types (6).MITF (D5G7V) Rabbit mAb recognizes endogenous levels of total MITF protein. The an-tibody is predicted to recognize all known isoforms of MITF. Based on sequence similarities, this antibody may also detect the related MiT/TEF protein family member TFE3.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using MITF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H +L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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