MKL1

Western blot analysis of extracts of various cell lines, using MKL1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using MKL1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:AB-84312
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids1-280 of human MKL1.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50
Molecular Weight:145kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MKL1; BSAC; MAL; MRTF-A

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids1-280 of human MKL1.

Target Background

The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator ofsmooth muscle cell differentiation. The encoded protein is predominantly nuclear and may helptransduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocationevent that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocationhas been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.

Synonyms:MKL1; BSAC; MAL; MRTF-A

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using MKL1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human tonsil using MKL1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U-2 OS cell susing MKL1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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