Immunological Sciences

MLH1 Mouse Monoclonal Antibody

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Catalog Number:MABN82444
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Purified recombinant fragment of human MLH1 (AA:381-483) expressed in E. Coli.
Reactivity:Human
Applications:WB 1:500-1:2000,IHC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400
Molecular Weight:84.6kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:FCC2; COCA2; HNPCC; hMLH1; HNPCC2

Applications

WB 1:500-1:2000,IHC 1:200-1:1000,ELISA 1:5000-1:20000,FC 1:200-1:400

Immunogen

Purified recombinant fragment of human MLH1 (AA:381-483) expressed in E. Coli.

Target Background

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Synonyms:FCC2; COCA2; HNPCC; hMLH1; HNPCC2

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide

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