Immunological Sciences

MMP-9 / Gelatinase-B Rabbit Polyclonal Antibody

Western Blot analysis of HepG2 cells using MMP-9 Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HepG2 cells using MMP-9 Polyclonal Antibody diluted at 1:1000

Catalog Number:AB-83613
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human MMP-9. AA range:651-700
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry(paraffin-embedded tissues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000
Molecular Weight:78kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry(paraffin-embedded tissues): 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000

Immunogen

The antiserum was produced against synthesized peptide derived from human MMP-9. AA range:651-700

Target Background

matrix metallopeptidase 9(MMP9) Homo sapiens embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as

Synonyms:MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of HepG2 cells using MMP-9 Polyclonal Antibody diluted at 1:1000

Review: Experiment Type Western blot (WB) Sample Human cells Description Western blot analysis of extracts of Human cells, using MMP9 Polyclonal Antibody.

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, GoatAnti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min). 3, Picture B: DAPI(blue) 10min.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-9 Antibody. The picture on the right is blocked with the synthesized peptide.

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