MMP9 Polyclonal Antibody
Western blot analysis of lysates from U-2 OS cells usingMMP9 Rabbit pAb at 1:5000 dilution incubatedat room temperature for 1.5 hours. U-2 OS cells were treated with PMA (200 nM) at 37℃ for 48 hours after serumstarvation overnight.|Secondary antibody: HRP-conjugatedGoat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3% nonfatdry milk in TBST.| Detection: ECL West Pico Plus. Exposure time: 20 s.
| Catalog Number: | AB93037 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Rabbit |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein of human MMP9 |
| Reactivity: | Human |
| Applications: | WB: 1:1000-1:5000 IHC 1:50-1:200; IF 1:50-1:200 |
| Molecular Weight: | 92 kDa / Glycosylated Form: ~106kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Depending on the cell type and species (e.g., in mice), glycosylation may cause the bands to migrate slightly higher, between 90kDa and 105 kDa.
Applications
WB: 1:1000-1:5000 IHC 1:50-1:200; IF 1:50-1:200
Immunogen
Recombinant fusion protein of human MMP9
