MMP9 Rabbit Polyclonal Antibody

Immunohistochemistry of paraffinembedded rat spleen using MMP9 antibody  at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded rat spleen using MMP9 antibody  at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Catalog Number:AB-84739
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesizedpeptide derived from human MMP-9. AA range:651-700
Reactivity:Human;Mouse;Rat
Applications:IWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300
Molecular Weight:78kDa
Purification:Affinity purification
Form:Liquid
Synonyms:MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

matrix metallopeptidase 9(MMP9) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008],

Applications

IWestern Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300

Immunogen

The antiserum was produced against synthesizedpeptide derived from human MMP-9. AA range:651-700

Target Background

matrix metallopeptidase 9(MMP9) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissueremodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secretedas inactive proproteins which are activated whencleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilizationof hematopoietic progenitor cells frombone marrow, and murine studies suggest a role in tumor-associated tissue remodeling

Synonyms:MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide.

Immunohistochemistry of paraffinembedded rat spleen using MMP9 antibody  at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded mouse testis using MMP9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded mouse spleen using MMP9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of L929 cells using MMP9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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