MOG /Myelin oligodendrocyte glycoprotein

Western blot analysis of extracts of various cell lines, using Myelin oligodendrocyte glycoprotein
antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.
Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using Myelin oligodendrocyte glycoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Catalog Number:MAB-94594
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC0879
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Myelin oligodendrocyte glycoprotein
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:28kDa
Purification:Affinity purification
Form:Liquid
Synonyms:BTN6; BTNL11; MOGIG2; NRCLP7

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Myelin oligodendrocyte glycoprotein

Target Background

The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms:BTN6; BTNL11; MOGIG2; NRCLP7

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Myelin oligodendrocyte glycoprotein antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.

Immunofluorescence analysis of rat brain using Myelin oligodendrocyte glycoprotein antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse brain using Myelin oligodendrocyte glycoprotein antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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