Mouse anti Strep II-Tag mAb
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Strep II-tagged protein using Mouse anti Strep II-Tag mAb . Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10 s.
| Catalog Number: | MAB066 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Host: | Mouse |
| Isotype: | IgG1,Kappa |
| Clone: | GT661 |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Reactivity: | Species independent |
| Applications: | WB 1:2000 - 1:5000; ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 32kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | Strep II;Strep II tag;Strep II-tag |
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes. Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Applications
WB 1:2000 - 1:5000; ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.