Immunological Sciences

mTOR

Western blot analysis of extracts of HeLa cells, using mTOR antibody dilution: 1:500. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of HeLa cells, using mTOR antibody dilution: 1:500. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:MAB-94142
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D9C2
Immunogen:Recombinant protein of human MTOR
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50 Immunofluorescence: 1:50 Immunoprecipitation: 1:20
Molecular Weight:288kDa
Purification:Aff, Pur.
Form:Liquid

Applications

Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50 Immunofluorescence: 1:50 Immunoprecipitation: 1:20

Immunogen

Recombinant protein of human MTOR

Target Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinaserelated kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of HeLa cells, using mTOR antibody dilution: 1:500. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

No documents available.