Immunological Sciences

mTOR

Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Femto Plus Exposure time: 15s

Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Femto Plus Exposure time: 15s

Catalog Number:AB-83580
Conjugate:Unconjugated
Size:100 ul
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR
Reactivity:Hu,Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Immunoprecipitation: 1:50
Molecular Weight:Calculated MW: 288kDa Observed MW: 290kDa
Purification:Aff, Pur.
Form:Liquid
Synonyms:MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Immunoprecipitation: 1:50

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR

Target Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Synonyms:MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS; mechanistic target of rapamycin

Storage

At +4°C for short term. For longer term store at -20°C, avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Femto Plus Exposure time: 15s

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody Western blot was performed from the immunoprecipitate using mTOR antibody at a dil. of 1:100

Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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