Immunological Sciences

Myocilin Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using MYOC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using MYOC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-J1705
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-504 of human MYOC.
Reactivity:Human, Mouse
Applications:Western Blot: 1:500 - 1:2000
Molecular Weight:65kDa
Purification:Affinity purification
Form:Liquid
Synonyms:GPOA; JOAG; TIGR; GLC1A; JOAG1; MYOC

Applications

Western Blot: 1:500 - 1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-504 of human MYOC.

Target Background

MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.

Synonyms:GPOA; JOAG; TIGR; GLC1A; JOAG1; MYOC

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using MYOC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

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