Myogenin Rabbit Polyclonal Antibody

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit 
Exposure time: 30s.

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30s.

Catalog Number:AB6664
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:Recombinant protein.This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:34kDa
Purification:Affinity purification
Form:liquid
Synonyms:MYF4; myf-4; bHLHc3; Myogenin

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.

Applications

WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant protein.This information is considered to be commercially sensitive.

Target Background

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.

Synonyms:MYF4; myf-4; bHLHc3; Myogenin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of lysates from Rat skeletal muscle, using Myogenin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 30s.

Western blot analysis of various lysates, using Myogenin Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): 293T,NIH/3T3 Exposure time: 30s.

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