NANOG Mouse Monoclonal Antibody

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Catalog Number:MABN80700
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Reactivity:Human
Applications:WB 1:500-1:2000,ICC 1:200-1:1000,ELISA 1:5000-1:20000
Molecular Weight:35kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:NANOG

Applications

WB 1:500-1:2000,ICC 1:200-1:1000,ELISA 1:5000-1:20000

Immunogen

Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Target Background

NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Synonyms:NANOG

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide.

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