NCE2 Rabbit Monoclonal Antibody

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Catalog Number:MABN02307
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:Recombinant protein of human NCE2
Reactivity:Human
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200
Molecular Weight:Calculated MW: 21 kDa; Observed MW: 21 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200

Immunogen

Recombinant protein of human NCE2

Target Background

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Synonyms:NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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