NEFM Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Catalog Number:MABN81800
Conjugate:Unconjugated
Size:100ul
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.
Reactivity:Human
Applications:ICC 1:50-1:200,ELISA 1:5000-1:20000,FC 1:200-1:400
Molecular Weight:5kDa
Purification:Affinity purification
Form:liquid
Synonyms:NFM; NEF3; NF-M

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Applications

ICC 1:50-1:200,ELISA 1:5000-1:20000,FC 1:200-1:400

Immunogen

Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.

Target Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms:NFM; NEF3; NF-M

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunofluorescence analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).

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