Immunological Sciences

NELFE Rabbit Monoclonal Antibody

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Catalog Number:MABN85848
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:Recombinant protein of human NELFe
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IHC 1:50-1:100,IP 1:10-1:20
Molecular Weight:Calculated MW: 43 kDa; Observed MW: 43 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:RD; RDP; RDBP; D6S45; NELF-E

Applications

WB 1:500-1:1000,IHC 1:50-1:100,IP 1:10-1:20

Immunogen

Recombinant protein of human NELFe

Target Background

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Synonyms:RD; RDP; RDBP; D6S45; NELF-E

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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