Immunological Sciences

NeuN-FOX3

Western blot analysis of cytosolic (cyt) and nuclear enriched (nuc) fractions of whole brain lysates using rabbit pAb to FOX3/ NeuN N-terminal peptide, FOX3-nt, dilution 1:1,000 in green: [1] protein molecular weight standard (red), [2] rat cyt, [3] rat nuc, [4] mouse cyt, and [5] mouse nuc lysate. Two bands of 46 and 48kDa correspond to the two alternate transcripts of the FOX3/NeuN protein. Western blotting was performed under non reducing conditions.

Western blot analysis of cytosolic (cyt) and nuclear enriched (nuc) fractions of whole brain lysates using rabbit pAb to FOX3/ NeuN N-terminal peptide, FOX3-nt, dilution 1:1,000 in green: [1] protein molecular weight standard (red), [2] rat cyt, [3] rat nuc, [4] mouse cyt, and [5] mouse nuc lysate. Two bands of 46 and 48kDa correspond to the two alternate transcripts of the FOX3/NeuN protein. Western blotting was performed under non reducing conditions.

Catalog Number:MAB-94416
Conjugate:Unconjugated
Size:100 UG
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D4G40
Immunogen:recombinant protein specific to theamino terminus of human NeuN protein.
Reactivity:Hu, Ms, Rt
Applications:WB: 1:1000, ICC, IF: 1:50, IHC: 1:400
Molecular Weight:46-55 kDa
Purification:Purified
Form:Liquid

Applications

WB: 1:1000, ICC, IF: 1:50, IHC: 1:400

Immunogen

recombinant protein specific to theamino terminus of human NeuN protein.

Target Background

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3).

Storage

4°C for short term and -20°C for longer term

Western blot analysis of cytosolic (cyt) and nuclear enriched (nuc) fractions of whole brain lysates using rabbit pAb to FOX3/ NeuN N-terminal peptide, FOX3-nt, dilution 1:1,000 in green: [1] protein molecular weight standard (red), [2] rat cyt, [3] rat nuc, [4] mouse cyt, and [5] mouse nuc lysate. Two bands of 46 and 48kDa correspond to the two alternate transcripts of the FOX3/NeuN protein. Western blotting was performed under non reducing conditions.

Confocal immunofluorescent analysis of mouse cerebellum using NeuN (D4G4O) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin (red). Blue pseudocolor = DRAQ5®4 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded mouse colon (myenteric plexus) using NeuN (D4G4O) Rabbit mAb.

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