Neurofilament Light (NF-L) Rabbit Polyclonal Antibody

Western Blot analysis of extracts from Jurkat cells, using NF-L Polyclonal Antibody. Secondary antibody  was diluted at 1:20000

Western Blot analysis of extracts from Jurkat cells, using NF-L Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Catalog Number:AB-84805
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from the C-terminal region of human NF-L.
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1/500 - 1/2000 Immunohistochemistry (paraffin embedded tissues): 1:100-300
Molecular Weight:61kD
Purification:Store at -20°C. Avoid repeated freeze-thaw cycles.
Form:Liquid
Synonyms:NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein.

Applications

Western Blot: 1/500 - 1/2000 Immunohistochemistry (paraffin embedded tissues): 1:100-300

Immunogen

Synthesized peptide derived from the C-terminal region of human NF-L.

Target Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Synonyms:NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein.

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of extracts from Jurkat cells, using NF-L Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.

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