Immunological Sciences

NF-kB p65/RelA Rabbit Monoclonal Antibody

Western blot analysis of various lysates using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Western blot analysis of various lysates using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:MAB22331
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:C22B4
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat,Monkey
Applications:WB 1:5000 - 1:20000 IHC-P 1:200 - 1:2000 IF/ICC 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-500μg extracts of whole cells ChIP 5μg antibody for 10μg-15μg of Chromatin ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:65kDa
Purification:Affinity purification
Form:liquid
Synonyms:p65; CMCU; NFKB3; AIF3BL3; lA
Download Datasheet

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Applications

WB 1:5000 - 1:20000 IHC-P 1:200 - 1:2000 IF/ICC 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-500μg extracts of whole cells ChIP 5μg antibody for 10μg-15μg of Chromatin ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive.

Target Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Synonyms:p65; CMCU; NFKB3; AIF3BL3; lA

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Western blot analysis of various lysates using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO)HeLa cells, using [KO Validated] NF-kB p65/RelA Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] NF-kB p65/RelA Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

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