NFkB p65 Mouse mAb
Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFκB p65 diluted at 1:1,500.
| Catalog Number: | ABE1111 |
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/mL |
| Host: | Mouse |
| Isotype: | IgG1 |
| Clone: | MB-9 |
| Immunogen: | Recombinant Protein |
| Reactivity: | Human, Rat, Mouse |
| Applications: | WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Molecular Weight: | 65kDa |
| Purification: | Affinity Purification |
| Form: | liquid |
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
Applications
WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500
Immunogen
Recombinant Protein
