NRF2 Rabbit Monoclonal Antibody

Western Blot - NFE2L2 antibody
of various cell lines, using NRF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer:3% nonfat dry milk in TBST
Detection: ECL West Pico Plus Kit
Exposure time: 60s.

Western Blot - NFE2L2 antibody of various cell lines, using NRF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST Detection: ECL West Pico Plus Kit Exposure time: 60s.

Catalog Number:MAB-94130
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:SP611
Immunogen:The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1:1000 Immunohistochemistry (paraffin tissues): 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunofluoresence: 1:50 - 1:200
Molecular Weight:75-100kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:1000 Immunohistochemistry (paraffin tissues): 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunofluoresence: 1:50 - 1:200

Immunogen

The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605

Target Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals . Multiple transcript variants encoding different isoforms have been characterized for this gene.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot - NFE2L2 antibody of various cell lines, using NRF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST Detection: ECL West Pico Plus Kit Exposure time: 60s.

Immunohistochemistry – NFE2L2 antibody of paraffin-embedded mouse leydig cells - dilution 1:100

Immunohistochemistry - NFE2L2 antibody of paraffin-embedded mouse kidney – dilution 1:100

Immunofluorescence analysis of HeLa cells using NFE2L2 antibody using at dilution of 1:100. Blue: DAPI for nuclear staining.

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