NRF2 Rabbit Polyclonal Antibody
Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by NRF2-siRNA (F:CAUUGAUGUUUCUGAUCUATT,R:UAGAUCAGAAACAUC AAUGTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4° over night, AF680 secondary antibody was diluted at 1:10000, 37° 1hour.
| Catalog Number: | AB-84017 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605. |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000 |
| Molecular Weight: | 75-100kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form: | Liquid |
| Synonyms: | NFE2L2; NRF2; Nuclear factor erythroid 2-relatedfactor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2 |
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],
Applications
Western Blot: 1/500 - 1/2000Immunohistochemistry: 1/100 - 1/300Immunofluorescence: 1/200 - 1/1000ELISA: 1/40000
Immunogen
The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605.
