OPN (Osteopontin)
Western blot analysis of various lysates using Osteopontin Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.
| Catalog Number: | AB19092 |
| Conjugate: | Unconjugated |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | ARC0471 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Osteopontin (P10451). |
| Reactivity: | Ms, Rt |
| Applications: | WB,1:1000 - 1:2000 |
| Molecular Weight: | 35kDa/65kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Applications
WB,1:1000 - 1:2000
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Osteopontin (P10451).