OPN /Osteopontin

Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500

Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500

Catalog Number:ABE3083
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310
Reactivity:Hu,Ms,Rt
Applications:WB,IHC,IF,ELISA
Molecular Weight:60kD
Purification:Affinity Purified
Form:Liquid
Synonyms:None

secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Applications

WB,IHC,IF,ELISA

Immunogen

The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310

Target Background

secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms:None

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5%BSAand0.02% sodium azide.

Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500

Western Blot analysis of 453 cells using OPN Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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