p44/p42 MAPK ERK1/ERK2

Review for ERK1 / ERK2 Mouse mAb
Experiment Type Western blot (WB)
Sample mouse cells

Review for ERK1 / ERK2 Mouse mAb Experiment Type Western blot (WB) Sample mouse cells

Catalog Number:MAB-94598
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG
Clone:FB-24
Immunogen:A synthetic peptide of human ERK1 / ERK2
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:39kDa
Purification:Aff. Pur
Form:Liquid

Applications

Western Blot: 1:1000 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide of human ERK1 / ERK2

Target Background

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Review for ERK1 / ERK2 Mouse mAb Experiment Type Western blot (WB) Sample mouse cells

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 5s.

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