Pan Phospho-Threonine Rabbit mAb
Western blot analysis of lysates from A-431, using Pan Phospho-Threonine Rabbit mAb (AP1422) at 1:1000 dilution. A 431 treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
| Catalog Number: | AB1422 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC61310 |
| Immunogen: | A synthetic phosphorylated peptide around of human Threonine. |
| Reactivity: | Human,Mouse,Rat,Other (Wide Range Predicted) |
| Applications: | WB,1:500 - 1:1000 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 10-250kDa |
| Purification: | Affinity purification |
| Form: | liquid |
Applications
WB,1:500 - 1:1000 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around of human Threonine.
