PE Anti-Human CD8 Antibody[UCHT-4]
![Staining of normal human peripheral blood cells with PE Anti-Human CD8 Antibody[UCHT-4] (filled gray histogram) or PE Mouse IgG1, κ Isotype Control (empty black histogram). Cells in the lymphocytes gate were used for analysis.](/storage/products/127187/pe-anti-human-cd8-antibodyucht-4-mab00427-img-1.jpg)
Staining of normal human peripheral blood cells with PE Anti-Human CD8 Antibody[UCHT-4] (filled gray histogram) or PE Mouse IgG1, κ Isotype Control (empty black histogram). Cells in the lymphocytes gate were used for analysis.
| Catalog Number: | MAB00427 |
| Conjugate: | PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). |
| Size: | 100Tests |
| Concentration: | 5 μL/Test |
| Host: | Mouse |
| Isotype: | Mouse IgG1, κ |
| Clone: | UCHT-4 |
| Reactivity: | Human |
| Applications: | FCM - Each lot of this antibody is quality control tested by flow cytometric analysis. <b style="color:#0070ca">The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood).</b> Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Form: | Liquid |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck
Applications
FCM - Each lot of this antibody is quality control tested by flow cytometric analysis. <b style="color:#0070ca">The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood).</b> Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.