Immunological Sciences

PERK Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using PERK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Western blot analysis of extracts of various cell lines, using PERK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Catalog Number:MAB-94697
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:C33E10
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 580-720 of human PERK
Reactivity:Hu, Ms, Rt
Applications:WB 1:500 - 1:1000
Molecular Weight:140kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:EIF2AK3; PEK; PERK; WRS; eukaryotic translation initiation factor 2-alpha kinase 3

Applications

WB 1:500 - 1:1000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 580-720 of human PERK

Target Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.

Synonyms:EIF2AK3; PEK; PERK; WRS; eukaryotic translation initiation factor 2-alpha kinase 3

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using PERK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Western blot analysis of extracts of NIH/3T3 cells, using PERK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.

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