Immunological Sciences

PGC-1alpha

Western blot analysis of extracts of various cell lines, using PGC-1alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico ECL-2001. Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using PGC-1alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico ECL-2001. Exposure time: 90s.

Catalog Number:AB-83882
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PPARGC1A
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 Immunofluorescence: 1: 100
Molecular Weight:115Kda/120kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:PPARGC1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC- 1v; PGC1; PGC1A; PPARGC1; PPARG coactivator 1 alpha

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 Immunofluorescence: 1: 100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PPARGC1A

Target Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Synonyms:PPARGC1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC- 1v; PGC1; PGC1A; PPARGC1; PPARG coactivator 1 alpha

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using PGC-1alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico ECL-2001. Exposure time: 90s.

Immunofluorescence analysis of C6 cells using PGC-1alpha antibody) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PGC-1alpha antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using PGC-1alpha antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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