PHD1 Rabbit Monoclonal Antibody

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Catalog Number:MABN03270
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Reactivity:Human,Rat
Applications:WB 1:500-1:1000
Molecular Weight:Calculated MW: 44 kDa; Observed MW: 44 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:Estrogen-induced tag 6; HPH-3; PHD1

Applications

WB 1:500-1:1000

Target Background

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Synonyms:Estrogen-induced tag 6; HPH-3; PHD1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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