Phospho-ACCα (S80)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

Catalog Number:ABP-0298
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:The antiserum was produced against synthesized peptide derived between 46-95 amino acids from the N-Terminal region of human ACC1 around the phosphorylation site of Ser80.
Reactivity:Hu, Ms, Rt
Applications:Western blotting 1:500-1:3000 Immunohistochemistry (Paraffin) 1:150-100 Elisa: 1:1000
Molecular Weight:280 kDa
Purification:Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography.
Form:liquid

Applications

Western blotting 1:500-1:3000 Immunohistochemistry (Paraffin) 1:150-100 Elisa: 1:1000

Immunogen

The antiserum was produced against synthesized peptide derived between 46-95 amino acids from the N-Terminal region of human ACC1 around the phosphorylation site of Ser80.

Target Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCis the predominant isoform in liver, adipocytes and mammary gland, while the 280 kDa ACCis the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).Phospho-Acetyl-CoA Carbox-ylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79.

Storage

Store at –20°C. Do not aliquot the antibody.

Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or serum-starved (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody.

Western blot analysis of extracts from K562 cells treated with Insulin 0.01U/ml 15', using Acetyl CoA Carboxylase (Phospho-Ser80) Antibody. The lane on the right is treated with the synthesized peptide.

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