Immunological Sciences

Phospho-alpha Synuclein (S129) (15T7) Rabbit Monoclonal Antibody

Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate.

Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate.

Catalog Number:MABN05849
Conjugate:Unconjugated
Size:100 ug
Concentration:0.5mg/ml
Host:Rabbit
Isotype:IgG
Clone:15T7
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Ser129 of human alpha Synuclein
Reactivity:Human,Mouse,Rat
Applications:WB 1:1000-1:5000,IHC 1:200-1:500
Molecular Weight:14kDa
Purification:Affinity purification
Form:Liquid
Synonyms:NACP; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; SYN; SYUA; alpha-synuclein; PARK1; PARK4; PD1; SNCA
Download Datasheet

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Applications

WB 1:1000-1:5000,IHC 1:200-1:500

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser129 of human alpha Synuclein

Target Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Synonyms:NACP; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; SYN; SYUA; alpha-synuclein; PARK1; PARK4; PD1; SNCA

Storage

Aliquot and store at -20°C (valid for 12 months). Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-alpha Synuclein (S129) antibody. The section was pretreated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody(1/50) for 30 minutes at room temperature.

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