Immunological Sciences

Phospho-AMPKα1/2 (T183/172)

Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.

Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.

Catalog Number:ABP-0116
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα
Reactivity:Hu, Ms, Rt, Monkey
Applications:WB 1:1,000 IP: 1:50 IHC: 1:50
Molecular Weight:64kDa
Purification:Affinity purification
Form:liquid
Synonyms:AMPKa1/AMPKa2

Applications

WB 1:1,000 IP: 1:50 IHC: 1:50

Immunogen

A phospho specific peptide corresponding to residues surrounding T172 of human AMPKα

Target Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is thecatalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellularenergy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activatedby the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of anumber of key metabolic enzymes through phosphorylation. It protects cells fromstresses that cause ATP depletion by switching off ATP-consuming biosyntheticpathways. Alternatively spliced transcript variants encoding distinct isoforms have beenobserved. [provided by RefSeq, Jul 2008]

Synonyms:AMPKa1/AMPKa2

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKALPHA 1-T183/AMPKALPHA 2-T172 antibody at 1:2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Immunological Sciences. Exposure time: 1s.

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