Phospho-Beclin-1 (S93) Rabbit Monoclonal Antibody

Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Catalog Number:MAB-94690
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D9A5G
Immunogen:A synthetic phosphorylated peptide around S93 of human Beclin 1
Reactivity:Hu
Applications:WB 1:500 - 1:2000
Molecular Weight:60KDa
Purification:Affinity purification
Form:liquid
Synonyms:ATG6; VPS30; beclin1

Applications

WB 1:500 - 1:2000

Immunogen

A synthetic phosphorylated peptide around S93 of human Beclin 1

Target Background

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Synonyms:ATG6; VPS30; beclin1

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of extracts of HeLa cells, using Phospho-Beclin 1-Ser93 antibody at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

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