Immunological Sciences

Phospho-BRAF (Thr401) Rabbit Monoclonal antibody

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Catalog Number:MABN01548
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight:Calculated MW: 84 kDa; Observed MW: 84 kDa
Purification:Affinity Purified
Form:Liquid
Synonyms:BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Applications

WB 1:500-1:1000,IP 1:20-1:50

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf

Target Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

Synonyms:BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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