Immunological Sciences

Phospho-c-Myc (S62)

Western Blot (WB) analysis of 293T, AD293, 22RV1 and HeLa cells, using Anti-Phospho-c-Myc (S62) polyclonal antibody.

Western Blot (WB) analysis of 293T, AD293, 22RV1 and HeLa cells, using Anti-Phospho-c-Myc (S62) polyclonal antibody.

Catalog Number:ABP10261
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:Synthesized peptide derived from human c-Myc around the phosphorylation site of S62.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000
Molecular Weight:50 kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Form:liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:40000

Immunogen

Synthesized peptide derived from human c-Myc around the phosphorylation site of S62.

Target Background

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes.Phospho-c-Myc (S62) Polyclonal Antibody detects endogenous levels of c- Myc protein only when phosphorylated at S62.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Western Blot (WB) analysis of 293T, AD293, 22RV1 and HeLa cells, using Anti-Phospho-c-Myc (S62) polyclonal antibody.

Western Blot (WB) analysis of 293 cells using Anti-Phospho-c-Myc (S62) polyclonal antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer using Anti-Phospho-c-Myc (S62) polyclonal antibody. Antibody was diluted at 1:100 (4°C, overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antibody retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

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