Phospho-c-Src (Tyr529) Rabbit Polyclonal Antibody

Western Blot analysis of various cells using Phospho-c-Src (Tyr529) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of various cells using Phospho-c-Src (Tyr529) Polyclonal Antibody diluted at 1:2000

Catalog Number:ABP10298
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr529. AA range:487-536.
Reactivity:Human;Mouse;Rat;Monkey
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300
Molecular Weight:59kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300

Immunogen

The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr529. AA range:487-536.

Target Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Synonyms:SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Phospho-c-Src (Tyr529) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of COS7 cells using Phospho-c-Src (Tyr529) Polyclonal Antibody diluted at 1:2000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (Phospho-Tyr529) Antibody. The picture on the right is blocked with the phospho peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (Phospho-Tyr529) Antibody

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